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THRB Rabbit pAb (bs-41495R)  
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產品編號 bs-41495R
英文名稱 THRB Rabbit pAb
中文名稱 甲狀腺激素受體β抗體
別    名 Thyroid Hormone Receptor beta; Avian erythroblastic leukemia viral(v erb a) oncogene homolog 2; C ERBA 2; C ERBA BETA; c-erbA-2; c-erbA-beta; ERBA 2; ERBA BETA; ERBA2; Erythroblastic leukemia viral(v erb a) oncogene homolog 2 avian; generalized resistance to thyroid hormone; GRTH; MGC126109; MGC126110; NR1A2; Nuclear receptor subfamily 1 group A member 2; Oncogene ERBA2; PRTH; THB_HUMAN; THR 1; THR1; THRB 1; THRB 2; THRB2; Thyroid hormone nuclear receptor beta variant 1; Thyroid hormone receptor beta 1; Thyroid hormone receptor beta 2; Thyroid hormone receptor beta; Thyroid hormone receptor, beta(erythroblastic leukemia viral(v erb a) oncogene homolog 2, avian); THβ1; THβ2.  
研究領域 心血管  細胞生物  神經生物學  生長因子和激素  內分泌病  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human
產品應用 IHC-P=1:200-800,IHC-F=1:400-800,IF=1:100-500,Flow-Cyt=1ug/Test,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 53 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinant human THRB protein : 209-461/461 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Thyroid hormone receptors (TRs) are ligand-dependent transcription factors that mediate the biological activities of thyroid hormone (T3). Thyroid hormone receptor b2 (TRb2) is a high affinity receptor for triiodothyronine which belongs to the nuclear hormone receptor family and the NR1 subfamily. It is composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain. Defects in the receptor result in generalized thyroid hormone resistance (GTHR). GTHR is transmitted as an autosomal dominant trait, but an autosomal recessive form also exists. The disease is characterized by goiter, abnormal mental functions, increased susceptibility to infections, abnormal growth and bone maturation, tachycardia and deafness. GTHR patients also have high levels of circulating thyroid hormones (T3-T4), with normal or slightly elevated thyroid stimulating hormone.

Function:
Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.

SWISS:
P10828

Gene ID:
7068

Database links:

Entrez Gene: 7068 Human

Entrez Gene: 24831 Rat

Omim: 190160 Human

SwissProt: P10828 Human

SwissProt: P18113 Rat

Unigene: 187861 Human

Unigene: 728126 Human

Unigene: 88692 Rat



產品圖片
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (THRB) Polyclonal Antibody, Unconjugated (bs-41495R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (THRB) Polyclonal Antibody, Unconjugated (bs-41495R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control(black line):Hela. Primary Antibody (green line): Rabbit Anti-THRB antibody (bs-41495R) Dilution:1ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control(black line):Hela. Primary Antibody (green line): Rabbit Anti-THRB antibody (bs-41495R) Dilution:1ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control(black line):A431. Primary Antibody (green line): Rabbit Anti-THRB antibody (bs-41495R) Dilution:1ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control(black line):A431. Primary Antibody (green line): Rabbit Anti-THRB antibody (bs-41495R) Dilution:1ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Measured by its binding ability in a indirect ELISA. Immobilized Human THRB protein, His Tag (Cat. bs-41495P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Human THRB Antibody, the EC50 is 942.9 ng/mL.
Measured by its binding ability in a indirect ELISA. Immobilized Human THRB protein, His Tag (Cat. bs-41495P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Human THRB Antibody, the EC50 is 133.9 ng/mL.
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