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Alpha Tubulin (Acetyl-Lys40) Recombinant Rabbit mAb (bsm-60706R)  
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產(chǎn)品編號(hào) bsm-60706R
英文名稱 Alpha Tubulin (Acetyl-Lys40) Recombinant Rabbit mAb
中文名稱 乙酰化微管蛋白α/Tubulin α重組兔單抗
別    名 alpha tubulin 1; alpha-tubulin 1; Detyrosinated alpha Tubulin; FLJ30169; H2 alpha; TBA4A_HUMAN; TBA1A_HUMAN; TBA1B_HUMAN; Testis specific alpha tubulin; Testis-specific alpha-tubulin; TUBA 4A; TUBA1; Tuba4a; Tubulin alpha 1(testis specific); Tubulin alpha 1; Tubulin alpha 1 chain; Tubulin alpha 4a; Tubulin alpha 4A chain; Tubulin alpha-1 chain; Tubulin alpha-4A chain; Tubulin H2 alpha; Tubulin H2-alpha; TUBA4A; α tubulin.  
產(chǎn)品類型 乙酰化抗體 重組兔單抗 
研究領(lǐng)域 細(xì)胞生物  染色質(zhì)和核信號(hào)  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號(hào) R2A9
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:1000-4000,IHC-P=1:100-500,IHC-F=1:50-100,IF=1:25,ICC/IF=1:25
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 50 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Alpha Tubulin (Acetyl-Lys40): SD(Ac-K)TI 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]


Subcellular Location:
Cytoplasm, cytoskeleton.

Tissue Specificity:
Widely expressed. Overexpressed in Platelet, Brain, and Frontal cortex

SWISS:
P68366

Gene ID:
7277

Database links:

Entrez Gene: 7277 Human

Entrez Gene: 22145 Mouse

Entrez Gene: 316531 Rat

SwissProt: P68366 Human

SwissProt: P68368 Mouse

SwissProt: Q5XIF6 Rat



產(chǎn)品圖片
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:6000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Mouse brain, 2: Rat brain Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 50 kDa Observed MW: 55 kDa
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): MCF-7+serum starvation(14hr), (+): MCF-7+ sodium butyrate (50mM, 24hr) + trichostain A (500ng/ml, 4 hr) Protein loading quantity: 20 μg Exposure time: 10 s Predicted MW: 50 kDa Observed MW: 55 kDa
Tissue: Rat cerebellum Section type: Formalin-fixed & Paraffin embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60706R
Tissue: Human cerebrum Section type: Formalin-fixed & Paraffin embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60706R
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