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Mart-1 Mouse mAb (bsm-34121M)  
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50ul/1180.00元
100ul/1980.00元
大包裝/詢價

產品編號 bsm-34121M
英文名稱 Mart-1 Mouse mAb
中文名稱 黑色素瘤相關抗原單克隆抗體
別    名 MAR1_HUMAN; Melanoma antigen recognized by T-cells 1; MLANA; MART1; Antigen LB39-AA; Antigen SK29-AA; Protein Melan-A;  
研究領域 腫瘤  細胞生物  免疫學  腫瘤細胞生物標志物  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 F4D11
交叉反應 Human
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 13 kDa
檢測分子量
細胞定位 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Mart-1 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 PBS containing 50% Glycerol, 0.5% BSA and 0.02% Proclin300.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Melan A, a product of the MART-1 gene, is a melanocyte differentiation marker recognized by autologous cytotoxic T lymphocytes. Other melanoma-associated markers recognized by autologous cytotoxic T cells are reported to include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1 and GAGE-1. The analysis of these different molecules and their expression in individual melanomas may be of help in the study of their particular molecular roles in melanocyte differentiation and tumorigenesis.

SWISS:
Q16655

Gene ID:
2315

產品圖片
25 ug total protein per lane of various lysates (see on figure) probed with Mart-1 monoclonal antibody, unconjugated (bsm-34121M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mart-1 Monoclonal Antibody, Unconjugated (bsm-34121M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Mart-1 Monoclonal Antibody, Unconjugated (bsm-34121M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
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