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ACTH Mouse mAb (bsm-34147M)  
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產品編號 bsm-34147M
英文名稱 ACTH Mouse mAb
中文名稱 促腎上腺皮質激素單克隆抗體
別    名 Adrenocorticotropic hormone; Adrenocorticotropin; Adrenocorticotropin Hormone; alpha Melanocyte Stimulating Hormone; beta Lipotropin; beta Melanocyte Stimulating Hormone; CLIP; Corticotropin; Corticotropin Like Intermediary Peptide; Corticotropin lipotropin precursor; Lipotropin beta; Lipotropin gamma; LPH; Melanotropin alpha; Met Enkephalin; MSH; NPP; POC; POMC; Pro opiomelanocortin; Proopiomelanocortin; COLI_HUMAN.  
研究領域 生長因子和激素  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 7D13
交叉反應 Human
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 kDa
檢測分子量
細胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ACTH 
亞    型 IgG1, Kappa
純化方法 affinity purified by Protein A
緩 沖 液 Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Proclin300.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008].

Function:
ACTH stimulates the adrenal glands to release cortisol. MSH (melanocyte-stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin and Met-enkephalin are endogenous opiates.

Subunit:
Belongs to the POMC family.

Subcellular Location:
Secreted.

Tissue Specificity:
ACTH and MSH are produced by the pituitary gland.

Post-translational modifications:
Specific enzymatic cleavages at paired basic residues yield the different active peptides. O-glycosylated; reducing sugar is probably N-acetylgalactosamine.

DISEASE:
Defects in POMC may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD) [MIM:609734]. Affected individuals present early-onset obesity, adrenal insufficiency and red hair.

Similarity:
Belongs to the POMC family.

SWISS:
P01189

Gene ID:
5443

Database links:

Entrez Gene: 5443 Human

Entrez Gene: 18976 Mouse

Entrez Gene: 24664 Rat

Omim: 176830 Human

SwissProt: P01190 Cow

SwissProt: P01189 Human

SwissProt: P01193 Mouse

SwissProt: P01194 Rat



產品圖片
Paraformaldehyde-fixed, paraffin embedded (Human pituitary adenoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ACTH) Monoclonal Antibody, Unconjugated (bsm-34147M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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