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Prostatic acid phosphatase Mouse mAb (bsm-34159M)  
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50ul/1180.00元
100ul/1980.00元
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產品編號 bsm-34159M
英文名稱 Prostatic acid phosphatase Mouse mAb
中文名稱 前列腺酸性磷酸酶單克隆抗體
別    名 Prosaposin; A1 activator; Cerebroside sulfate activator; Co-beta-glucosidase; Component C; CSAct; Dispersin; GLBA; Glucosylceramidase activator; Proactivator polypeptide; Proactivator polypeptide precursor; Prosaposin(sphingolipid activator protein 1); prosaposin(variant Gaucher disease and variant metachromatic leukodystrophy); Protein A; Protein C; PSAP; SAP-1; SAP-2; SAP_HUMAN; SAP1; Saposin A; Saposin B; Saposin B Val; Saposin C; Saposin D; Saposin-D; Saposins; Sgp1; Sphingolipid activator protein 1; Sphingolipid activator protein 2; Sulfated glycoprotein 1; Sulfatide/GM1 activator.  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 3B9
交叉反應 Human (predicted: Mouse,Rat)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 41 kDa
檢測分子量
細胞定位 細胞膜 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Prostatic Acid Phosphatase 
亞    型 IgG2b, Kappa
純化方法 affinity purified by Protein A
緩 沖 液 PBS containing 50% Glycerol, 0.5% BSA and 0.02% Proclin300.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Prostatic Acid Phosphatase is Belongs to the histidine acid phosphatase family.Cellular localization;Secreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles.

Function:
A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.

Subunit:
Homodimer; dimer formation is required for phosphatase activity.

Subcellular Location:
Isoform 1: Secreted. Isoform 2: Cell membrane. Isoform 2: the cellular form also has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor. Acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling.

Tissue Specificity:
Highly expressed in the prostate, restricted to glandular and ductal epithelial cells.

Post-translational modifications:
N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides. Proteolytically cleaved in seminal fluid to produce several peptides. Peptide PAPf39, the most prominent, forms amyloid beta-sheet fibrils, SEVI (semen-derived enhancer of viral infection) which entrap HIV virions, attach them to target cells and enhance infection. SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea. Target cell attachment and enhancement of HIV infection is inhibited by surfen. Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection.

Similarity:
Belongs to the histidine acid phosphatase family.

SWISS:
P15309

Gene ID:
55

Database links:

Entrez Gene: 504700 Cow

Entrez Gene: 55 Human

Entrez Gene: 56318 Mouse

Entrez Gene: 56780 Rat

Omim: 171790 Human

SwissProt: A6H730 Cow

SwissProt: P15309 Human

SwissProt: Q8CE08 Mouse

SwissProt: P20646 Rat

Unigene: 433060 Human

Unigene: 19941 Mouse

Unigene: 40121 Rat



產品圖片
Paraformaldehyde-fixed, paraffin embedded (human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Prostatic acid phosphatase ) Monoclonal Antibody, Unconjugated (bsm-34159M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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