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GCLM Recombinant Rabbit mAb (bsm-54141R)  
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產品編號 bsm-54141R
英文名稱 GCLM Recombinant Rabbit mAb
中文名稱 GCLM重組兔單抗
別    名 Glutamate-Cysteine Ligase Modifier Subunit; gamma-Glutamylcysteine Synthetase Regulatory Subunit; Glutamate--Cysteine Ligase Modifier Subunit; gamma-ECS Regulatory Subunit; GCS Light Chain; GLCLR; Glutamate-Cysteine Ligase(gamma-Glutamylcysteine Synthetase), Regulatory(30.8kD); Glutamate-Cysteine Ligase Modifier Subunit delta2 Alternative Splicing; Glutamate--Cysteine Ligase Regulatory Subunit; Glutamate-Cysteine Ligase Regulatory Protein; Glutamate-Cysteine Ligase, Modifier Subunit; gamma-Glutamylcysteine Synthetase; GSC Light Chain; GSH0_HUMAN;  γ 谷氨酰半胱氨酸連接酶調節亞基
研究領域 腫瘤  細胞生物  免疫學  轉錄調節因子  合成與降解  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 4G5
交叉反應 Human,Mouse,Rat
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1:50-100,ICC/IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 31 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 A synthesized peptide derived from human GCLM: 201-260 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Function:
This protein is involved in step 1 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.

Subunit:
Heterodimer of a catalytic heavy chain and a regulatory light chain.

Subcellular Location:
Cytosol

Tissue Specificity:
In all tissues examined. Highest levels in skeletal muscle.

Similarity:
Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.

SWISS:
P48507

Gene ID:
2730

Database links:

Entrez Gene: 2730 Human

Entrez Gene: 14630 Mouse

Entrez Gene: 29739 Rat

Omim: 601176 Human

SwissProt: P48507 Human

SwissProt: O09172 Mouse

SwissProt: P48508 Rat

Unigene: 315562 Human

Unigene: 292676 Mouse

Unigene: 2460 Rat



產品圖片
25 ug total protein per lane of various lysates (see on figure) probed with GCLM monoclonal antibody, unconjugated (bsm-54141R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GCLM Monoclonal Antibody, Unconjugated(bsm-54141R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GCLM Monoclonal Antibody, Unconjugated(bsm-54141R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GCLM Monoclonal Antibody, Unconjugated(bsm-54141R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GCLM Monoclonal Antibody, Unconjugated(bsm-54141R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GCLM Monoclonal Antibody, Unconjugated(bsm-54141R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GCLM Monoclonal Antibody, Unconjugated(bsm-54141R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GCLM Monoclonal Antibody, Unconjugated(bsm-54141R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GCLM Monoclonal Antibody, Unconjugated(bsm-54141R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GCLM Monoclonal Antibody, Unconjugated(bsm-54141R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GCLM Monoclonal Antibody, Unconjugated(bsm-54141R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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