| 產品編號 | BIO0983SM |
| 英文名稱 | Anti-4-1BB & CD40 Reference Antibody (Gen1042 Biosimilar) |
| 別 名 | TNFRSF9 / 4-1BB / CD137 & TNFRSF5 / CD40; Gen1042 |
| 抗體來源 | |
| 克隆類型 | Monoclonal |
| 交叉反應 | Human |
| 產品應用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 145.37kDa |
| 性 狀 | Lyophilized |
| 亞 型 | IgG-like |
| 純化方法 | Protein A |
| 緩 沖 液 | 100 mM Pro-Ac 20mM Arg pH 5.0 |
| 保存條件 | -80°C for 2 years under sterile conditions； -20°C for 1 year under sterile conditions；Avoid repeated freeze-thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 | |
| 產品圖片 |
Co-incubation of Gen1042 with 4-1BB-NF-κB-Jurkat cells, then with the addition of huCD40-CHO-K cells for 6 hours. Bright-Lite was used to detect the fluorescent signal. As shown in fig, Gen1042 was able to activate the NF-κB signaling pathway, and the EC50 was 0.234 nM.
Gen1042 bound to huCD40-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Gen1042 bound to huCD40-CHO-K cells, and the EC50 was 4.535 nM.
Gen1042 bound to hu4-1BB-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Gen1042 bound to hu4-1BB-CHO-K cells, and the EC50 was 4.119 nM.
Gen1042 bound to CD40 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Gen1042 bound to hu-CD40-His, and the EC50 was 0.066 nM.
Gen1042 bound to 4-1BB protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Gen1042 bound to hu-4-1BB-His, and the EC50 was 0.015 nM.
The purity of Anti-4-1BB & CD40 Reference Antibody (Gen1042) is 96.80%, determined by SEC-HPLC.
Anti-4-1BB & CD40 Reference Antibody (Gen1042) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
