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Anti-PD-1 & VEGF Reference Antibody (Ivonescimab Biosimilar) (BIO1019SM)  
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100ug/詢價
1mg/詢價
5mg/詢價
大包裝/詢價

產品編號 BIO1019SM
英文名稱 Anti-PD-1 & VEGF Reference Antibody (Ivonescimab Biosimilar)
別    名 PDCD1 / PD-1 / CD279 & VEGF; Ivonescimab  
抗體來源
克隆類型 Monoclonal
交叉反應 Human
產品應用
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 201.12kDa
性    狀 Lyophilized
亞    型 IgG-ScFv
純化方法 Protein A
緩 沖 液 100 mM Pro-Ac 20mM Arg pH 5.0
保存條件 -80°C for 2 years under sterile conditions; -20°C for 1 year under sterile conditions;Avoid repeated freeze-thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹
產品圖片
Co-incubation of Ivonescimab with VEGF protein, then with the addition of VEGF-NF-AT-HEK293 cells and incubated for 6 hours. Bright-Lite was used to detect the fluorescent signal. As shown in fig, Ivonescimab can neutralize VEGF-165, and the IC50 was 7.957 nM.
Co-incubation of Ivonescimab with PD-1-NF-AT-Jurkat and CD3L-huPD-L1-CHO-K cells and incubated for 6 hours. Bright-Lite was used to detect the fluorescent signal. As shown in fig, Ivonescimab was able to block the PD-1/PD-L1 signaling pathway, and the EC50 was 7.957 nM.
Ivonescimab bound to huPD-1-Jurkat cells, and then rebounded to fluorescent secondary antibodies(Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Ivonescimab bound to huPD-1-Jurkat cells, and the EC50 was 6.274 nM.
Ivonescimab bound to VEGFA protein, and then rebounded to secondary antibodies(Anti-human-IgG-Fc-HRP) , and read OD450. As shown in fig, Ivonescimab bound to huVEGFA-His, and the EC50 was 0.021 nM.
Ivonescimab bound to PD-1 protein, and then rebounded to secondary antibodies(Anti-human-IgG-Fc-HRP) , and read OD450. As shown in fig, Ivonescimab bound to huPD-1-His, and the EC50 was 0.051 nM.
The purity of Anti-PD-1 & VEGF Reference Antibody (Ivonescimab)is 98.33%, determined by SEC-HPLC.
Anti-PD-1 & VEGF Reference Antibody (Ivonescimab) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The detected molecular weight of Anti-PD-1 & VEGF Reference Antibody (Ivonescimab) is 201.13 kDa.
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