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Anti-4-1BB & PD-L1 Reference Antibody (Acasunlimab Biosimilar) (BIO0997SM)  
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100ug/詢價
1mg/詢價
5mg/詢價
大包裝/詢價

產品編號 BIO0997SM
英文名稱 Anti-4-1BB & PD-L1 Reference Antibody (Acasunlimab Biosimilar)
別    名 TNFRSF9 / 4-1BB / CD137 & B7-H1 / PD-L1 / CD274; Acasunlimab  
抗體來源
克隆類型 Monoclonal
交叉反應 Human
產品應用
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 144.08kDa
性    狀 Lyophilized
亞    型 IgG-like
純化方法 Protein A
緩 沖 液 100 mM Pro-Ac 20mM Arg pH 5.0
保存條件 -80°C for 2 years under sterile conditions; -20°C for 1 year under sterile conditions;Avoid repeated freeze-thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹
產品圖片
Co-incubation of Acasunlimab with PD-1-NF-AT-Jurkat and CD3L-huPD-L1-CHO-K cells and incubated for 6 hours. Bright-Lite was used to detect the fluorescent signal. As shown in fig, Acasunlimab was able to block the PD-1/PD-L1 signaling pathway, and the EC50 was 1.875 nM.
Co-incubation of Acasunlimab with 4-1BB-NF-κB-Jurkat cells, then with the addition of huPD-L1-CHO-K cells for 6 hours. Bright-Lite was used to detect the fluorescent signal. As shown in fig, Acasunlimab was able to activate the NF-κB signaling pathway, and the EC50 was 0.341 nM.
Acasunlimab bound to huPD-L1-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Acasunlimab bound to huPD-L1-CHO-K cells, and the EC50 was 1.153 nM.
Acasunlimab bound to hu4-1BB-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Acasunlimab bound to hu4-1BB-CHO-K cells, and the EC50 was 3.592 nM.
To measure the binding ability of Acasunlimab in hu-PD-L1-His. Acasunlimab bound to PD-L1 protein, and then rebounded to secondary antibodies(Anti-human-IgG-Fc-HRP) , and read OD450. As shown in fig, Acasunlimab bound to hu-PD-L1-His, and the EC50 was 0.042 nM.
To measure the binding ability of Acasunlimab in hu-4-1BB-His. Acasunlimab bound to 4-1BB protein, and then rebounded to secondary antibodies(Anti-human-IgG-Fc-HRP) , and read OD450. As shown in fig, Acasunlimab bound to hu-4-1BB-His, and the EC50 was 0.007 nM.
The purity of Anti-4-1BB & PD-L1 Reference Antibody (Acasunlimab) is 94.41%, determined by SEC-HPLC.
Anti-4-1BB & PD-L1 Reference Antibody (Acasunlimab) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
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