產品編號 | BIO1000SM |
英文名稱 | Anti-LAG-3 & PD-1 Reference Antibody (Tebotelimab Biosimilar) |
別 名 | LAG3 / CD223 & PDCD1 / PD-1 / CD279; Tebotelimab |
抗體來源 | |
克隆類型 | Monoclonal |
交叉反應 | Human |
產品應用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 165.68kDa |
性 狀 | Lyophilized |
亞 型 | DART-DART-Fc |
純化方法 | Protein A |
緩 沖 液 | 100 mM Pro-Ac 20mM Arg pH 5.0 |
保存條件 | -80°C for 2 years under sterile conditions; -20°C for 1 year under sterile conditions;Avoid repeated freeze-thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 | |
產品圖片 |
Co-incubation of Tebotelimab with PD-1-NF-AT-Jurkat and CD3L-huPD-L1-CHO-K cells and incubated for 6 hours. Bright-Lite was used to detect the fluorescent signal. As shown in fig, Tebotelimab was able to block the PD-1/PD-L1 signaling pathway, and the EC50 was 1.266 nM.
Tebotelimab bound to huPD-1-Jurkat cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Tebotelimab bound to huPD-1-Jurkatt cells, and the EC50 was 0.47 nM.
Tebotelimab bound to huLAG-3-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Tebotelimab bound to huLAG-3-CHO-K cells, and the EC50 was 8.727 nM.
Tebotelimab bound to huPD-1-Jurkat cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Tebotelimab bound to huPD-1-Jurkatt cells, and the EC50 was 0.47 nM.
Tebotelimab bound to huLAG-3-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Tebotelimab bound to huLAG-3-CHO-K cells, and the EC50 was 8.727 nM.
Anti-LAG-3 & PD-1 Reference Antibody (Tebotelimab) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
|
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |