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Anti-CTLA4 & PD-1 Reference Antibody (Vudalimab Biosimilar) (BIO1003SM)  
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100ug/詢價
1mg/詢價
5mg/詢價
大包裝/詢價

產(chǎn)品編號 BIO1003SM
英文名稱 Anti-CTLA4 & PD-1 Reference Antibody (Vudalimab Biosimilar)
別    名 CTLA-4 / CD152 & PDCD1 / PD-1 / CD279; Vudalimab  
抗體來源
克隆類型 Monoclonal
交叉反應(yīng) Human
產(chǎn)品應(yīng)用
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 125.43kDa
性    狀 Lyophilized
亞    型 Half IgG+ScFv
純化方法 Protein A
緩 沖 液 100 mM Pro-Ac 20mM Arg pH 5.0
保存條件 -80°C for 2 years under sterile conditions; -20°C for 1 year under sterile conditions;Avoid repeated freeze-thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹
產(chǎn)品圖片
Co-incubation of Vudalimab with PD-1-NF-AT-Jurkat and CD3L-huPD-L1-CHO-K cells and incubated for 6 hours. Bright-Lite was used to detect the fluorescent signal. As shown in fig, Vudalimab was able to block the PD-1/PD-L1 signaling pathway.
Vudalimab bound to huPD-1-Jurkat cells, and then rebounded to fluorescent secondary antibodies(Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Vudalimab bound to huPD-1-Jurkat cells, and the EC50 was 2.508 nM.
Vudalimab bound to CTLA4-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Vudalimab bound to CTLA4-CHO-K cells, and the EC50 was 20.150 nM.
Vudalimab bound to PD-1 protein, and then rebounded to secondary antibodies(Anti-human-IgG-Fc-HRP) , and read OD450. As shown in fig, Vudalimab bound to huPD-1-His, and the EC50 was 0.090 nM.
Vudalimab bound to CTLA4 protein, and then rebounded to secondary antibodies(Anti-human-IgG-Fc-HRP) , and read OD450. As shown in fig, Vudalimab bound to hu-CTLA4-His, and the EC50 was 0.480 nM.
The purity of Anti-CTLA4 & PD-1 Reference Antibody (Vudalimab) is 98.25%, determined by SEC-HPLC.
Anti-CTLA4 & PD-1 Reference Antibody (Vudalimab) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The detected molecular weight of Anti-CTLA4 & PD-1 Reference Antibody (Vudalimab) is 125.32 kDa.
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