| 產品編號 | bs-1563R-Bio |
| 英文名稱 | Rabbit Anti-E.coli O157:H7/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的腸出血性大腸桿菌埃希氏菌O157:H7抗體 |
| 別 名 | Escherichia coli O157:H7; Enterohemorrhage E．Coli; EHEC |
| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 免疫學 細菌及病毒 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Escherichia Coli, ) |
| 產品應用 | ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Ecoli O157;H7 protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: This antibody reacts with Escherichia coli O157 (E.coli O157), Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins. Function: Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ????EHEC-腸出血性大腸埃希氏菌O157:H7(E.coli O157:H7)為產腸毒素大腸桿菌埃希氏菌-腸出血致病性大腸埃希氏菌的一種與志賀痢疾桿菌I型產生的毒素相似。 ????EHEC主要侵犯結腸，在腸道能使腸粘膜細胞壞死，引起組織病變-形成腸壁潰瘍； EHEC-大腸埃希氏菌O157：H7是毒力最強的一種，該毒株對很多抗菌素都有不同的耐、抗藥性，嚴重者，可導致溶血性尿毒綜合征癥狀,治療不及時會引起死亡。 ????EHEC引起腸道侵襲性病變，主要在結腸部位。研究認為ETEC產生的志賀樣毒素與腸壁組織的微血管內皮細胞上的受體結合，引起血管損傷，同時激發局部細胞因子TNF、LPS及IL1β增加。這些細胞因子又增強受體對毒素的敏感性，促使血管損傷進一步加重。此毒素是否作用于其他臟器內皮細胞，激發產生細胞因子，引起局部損傷-引起血管出血情況的現象有待進一步研究。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
