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Rabbit Anti-LAMP1/FITC Conjugated antibody (bs-1970R-FITC)
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說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價

產品編號 bs-1970R-FITC
英文名稱 Rabbit Anti-LAMP1/FITC Conjugated antibody
中文名稱 FITC標記的溶酶體相關膜蛋白1抗體
別    名 CD107 antigen like family member A; CD107 antigen-like family member A; CD107a; CD107a antigen; LAMP 1; LAMP-1; LAMP1_HUMAN; LAMPA; LGP120; lgpA; Lysosomal membrane glycoprotein 120KD; Lysosomal Associated Membrane Protein 1; Lysosome associated membrane glycoprotein 1; Lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1; OTTHUMP00000040663.  
Journal
PMID
IF
Application
[IF=13.751] Ji Eun Won. et al. Immune checkpoint silencing using RNAi-incorporated nanoparticles enhances antitumor immunity and therapeutic efficacy compared with antibody-based approaches. J Immunother Cancer. 2022 Feb;10(2):e003928  FC ;  Mouse.  
規格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
研究領域 腫瘤  免疫學  神經生物學  信號轉導  干細胞  細胞類型標志物  新陳代謝  細胞膜蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, 
產品應用 Flow-Cyt=2ug/Test IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 42kDa
細胞定位 細胞膜 
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human LAMP-1
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹 background:
Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation.

Function:
Presents carbohydrate ligands to selectins. Also mplicated in tumor cell metastasis.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane.

Post-translational modifications:
O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.

Similarity:
Belongs to the LAMP family.

Database links:

Entrez Gene: 3916 Human

Entrez Gene: 16783 Mouse

Entrez Gene: 25328 Rat

Omim: 153330 Human

SwissProt: P11279 Human

SwissProt: P11438 Mouse

SwissProt: P14562 Rat

Unigene: 494419 Human

Unigene: 16716 Mouse

Unigene: 475822 Mouse

Unigene: 40177 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CD107a可很好的反映NK細胞活性,其表達水平的上調與NK分泌細胞因子的增多及NK對靶細胞的殺傷作用的增強高度相關,采用流式檢測,CD107a還可鑒定出一群無細胞因子分泌的活性NK細胞。
產品圖片
Blank control: Jurkat. Primary Antibody (green line): Rabbit Anti-LAMP1 antibody (bs-1970R-FITC) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:Jurkat. Primary Antibody (green line): Rabbit Anti-LAMP1 antibody (bs-1970R-FITC) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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